The bidirectional upstream element of the adenovirus-2 major late promoter binds a single monomeric molecule of the upstream factor.
AUTOR(ES)
Lennard, A C
RESUMO
The adenovirus-2 major late promoter (Ad2MLP) upstream element (Ad2MLP-UE) contains a sequence of interrupted dyad symmetry. By inverting this element we have found that it functions in a bidirectional manner both in vivo and in vitro. Footprinting and binding kinetics studies have demonstrated that both orientations of the upstream element bind the sequence-specific upstream factor (UEF) in a similar fashion. These data strongly suggest that the dyad symmetric sequence is sufficient for fully functional binding of the UEF. Binding studies of the UEF to the Ad2MLP-UE indicate that, contrary to prokaryotic palindromic promoter elements which bind multimers of specific factors, the entire Ad2MLP dyad symmetric upstream element binds a single monomeric UEF molecule.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=553739Documentos Relacionados
- Stimulation of in vitro transcription by the upstream element of the adenovirus-2 major late promoter involves a specific factor.
- Specific interaction between a transcription factor and the upstream element of the adenovirus-2 major late promoter.
- Multiple transcription factors interact with the adenovirus-2 EII-late promoter: evidence for a novel CCAAT recognition factor.
- The mammalian upstream element factor recognizes two sites in the adenovirus type 2 IVa2-major late promoter intergenic region and stimulates both promoters.
- A single polypeptide possesses the binding and transcription activities of the adenovirus major late transcription factor.
