The BldD Protein from Streptomyces coelicolor Is a DNA-Binding Protein

AUTOR(ES)

Elliot, Marie A.

FONTE

American Society for Microbiology

RESUMO

Gel mobility shift assays with His-tagged BldD isolated from Escherichia coli have illustrated that BldD is capable of specifically recognizing its own promoter region. DNase I and hydroxyl radical footprinting assays have served to delimit the BldD binding site, revealing that BldD recognizes and binds to a site just upstream from, and overlapping with, the −10 region of the promoter. How BldD binds to its promoter and the effect this binding has on the expression of BldD are discussed.

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