The C.elegans ric-3 gene is required for maturation of nicotinic acetylcholine receptors
AUTOR(ES)
Halevi, Sarah
FONTE
Oxford University Press
RESUMO
Mutations in ric-3 (resistant to inhibitors of cholinesterase) suppress the neuronal degenerations caused by a gain of function mutation in the Caenorhabditis elegans DEG-3 acetylcholine receptor. RIC-3 is a novel protein with two transmembrane domains and extensive coiled-coil domains. It is expressed in both muscles and neurons, and the protein is concentrated within the cell bodies. We demonstrate that RIC-3 is required for the function of at least four nicotinic acetylcholine receptors. However, GABA and glutamate receptors expressed in the same cells are unaffected. In ric-3 mutants, the DEG-3 receptor accumulates in the cell body instead of in the cell processes. Moreover, co-expression of ric-3 in Xenopus laevis oocytes enhances the activity of the C.elegans DEG-3/DES-2 and of the rat α-7 acetylcholine receptors. Together, these data suggest that RIC-3 is specifically required for the maturation of acetylcholine receptors.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=125878Documentos Relacionados
- Regulation of the mec-3 gene by the C.elegans homeoproteins UNC-86 and MEC-3.
- The C.elegans MAPK phosphatase LIP-1 is required for the G2/M meiotic arrest of developing oocytes
- Cloning and characterization of the C.elegans histidyl-tRNA synthetase gene.
- Distinct requirements for C.elegans TAFIIs in early embryonic transcription
- Related transposons in C.elegans and D.melanogaster.