The CDK regulates repair of double-strand breaks by homologous recombination during the cell cycle
AUTOR(ES)
Aylon, Yael
FONTE
Nature Publishing Group
RESUMO
DNA double-strand breaks (DSBs) are dangerous lesions that can lead to genomic instability and cell death. Eukaryotic cells repair DSBs either by nonhomologous end-joining (NHEJ) or by homologous recombination. We investigated the ability of yeast cells (Saccharomyces cerevisiae) to repair a single, chromosomal DSB by recombination at different stages of the cell cycle. We show that cells arrested at the G1 phase of the cell cycle restrict homologous recombination, but are able to repair the DSB by NHEJ. Furthermore, we demonstrate that recombination ability does not require duplicated chromatids or passage through S phase, and is controlled at the resection step by Clb–CDK activity.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=535085Documentos Relacionados
- Homologous recombination and the repair of double-strand breaks during cotransformation of Dictyostelium discoideum.
- Repair of Double-Strand Breaks by Homologous Recombination in Mismatch Repair-Defective Mammalian Cells
- Repair of site-specific double-strand breaks in a mammalian chromosome by homologous and illegitimate recombination.
- Efficient Repair of Genomic Double-Strand Breaks by Homologous Recombination between Directly Repeated Sequences in the Plant Genome
- Pathways of DNA Double-Strand Break Repair during the Mammalian Cell Cycle