The chromosomal protein MC1 from the archaebacterium Methanosarcina sp. CHTI 55 induces DNA bending and supercoiling.
AUTOR(ES)
Laine, B
RESUMO
We have investigated the effect on the DNA structure of protein MC1, a basic and small polypeptide (Mr 10700) representing the major chromosomal protein in Methanosarcinaceae. The ability of protein MC1 to strongly favour cyclization upon polymerization of short DNA fragments by T4 DNA ligase indicates that protein MC1 mediates DNA bending. Several negatively supercoiled topoisomers of minicircles were obtained with DNA fragments of 203 and 146 bp, their distribution depends upon the amount of protein MC1 complexed with DNA. In addition, protein MC1 can induce a compaction of a nicked plasmid.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=328268Documentos Relacionados
- Common sequence determinants of the response of a prokaryotic promoter to DNA bending and supercoiling.
- Gyrase inhibitors can increase gyrA expression and DNA supercoiling.
- Electron microscopic studies of the interaction between a Bacillus subtilis alpha/beta-type small, acid-soluble spore protein with DNA: protein binding is cooperative, stiffens the DNA, and induces negative supercoiling.
- Regulation of the Escherichia coli DNA topoisomerase I gene by DNA supercoiling.
- Regulation of the Escherichia coli nrd operon: role of DNA supercoiling.
