The dose of a putative ubiquitin-specific protease affects position-effect variegation in Drosophila melanogaster.

AUTOR(ES)

Henchoz, S

RESUMO

A dominant insertional P-element mutation enhances position-effect variegation in Drosophila melanogaster. The mutation is homozygous, viable, and fertile and maps at 64E on the third chromosome. The corresponding gene was cloned by transposon tagging. Insertion of the transposon upstream of the open reading frame correlates with a strong reduction of transcript level. A transgene was constructed with the cDNA and found to have the effect opposite from that of the mutation, namely, to suppress variegation. Sequencing of the cDNA reveals a large open reading frame encoding a putative ubiquitin-specific protease (Ubp). Ubiquitin marks various proteins, frequently for proteasome-dependent degradation. Ubps can cleave the ubiquitin part from these proteins. We discuss the link established here between a deubiquitinating enzyme and epigenetic silencing processes.

Documentos Relacionados

• Single-Locus Modification of Position-Effect Variegation in DROSOPHILA MELANOGASTER. I. White Variegation
• Mutation in a heterochromatin-specific chromosomal protein is associated with suppression of position-effect variegation in Drosophila melanogaster.
• Single-Locus Modification of Position-Effect Variegation in DROSOPHILA MELANOGASTER. II. Region 3c Loci
• Position-Effect Variegation at Several Closely Linked Loci in Drosophila Melanogaster
• Mutations in the Drosophila Melanogaster Gene Encoding S-Adenosylmethionine Suppress Position-Effect Variegation