The duplicated human alpha globin genes lie close together in cellular DNA.
AUTOR(ES)
Orkin, S H
RESUMO
The organization of alpha globin genes in normal human DNA was examined by restriction endonuclease mapping, alpha globin-specific fragments in endonuclease digests of total cell DNA were identified after electrophoresis by hybridization with [32P]cDNA following the blotting procedure of Southern [(1975) J. Mol. Biol. 98, 503--517]. The data provide direct evidence for the duplication of alpha genes and further indicate that these loci are closely linked within a single restriction fragment. The HindIII sites (codons 90/91) of these duplicated genes lie approximately 3.7 kilobases apart in the physical map proposed for this region. This organization of alpha genes can be altered in DNA of individuals with alpha-thalassemia.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=393094Documentos Relacionados
- Relative numbers of human globin genes assayed with purified alpha and beta complementary human DNA.
- Genes for gamma-globin in human adult erythroid DNA.
- Independent recombination events between the duplicated human alpha globin genes; implications for their concerted evolution.
- Highly variable regions of DNA flank the human alpha globin genes.
- The orangutan adult alpha-globin gene locus: duplicated functional genes and a newly detected member of the primate alpha-globin gene family.