The effect of hydroxyurea on the expression of the common fragile site at 3p14.
AUTOR(ES)
Yan, Z A
RESUMO
Hydroxyurea (HU) is an inhibitor of DNA synthesis, which can inhibit the enzyme ribonucleotide reductase, reduce the syntheses of all four deoxyribonucleoside diphosphates (dNDP), and disturb the balance of the dNTP pool. We have studied the effect of HU on the common fragile site at 3p14 (FRA3B) and have found that G2 treatment with HU increased not only the frequency of chromosomal aberration but also the expression of FRA3B in both complete and folate deficient media. There is a synergistic effect between HU and growth in folate deficient medium on the induction of FRA3B. Our results suggest that the inhibition of DNA repair, including the inhibition in G2 phase, plays an important role in the expression of FRA3B, supporting other authors' data on the effect of other DNA repair inhibitors, such as aphidicolin, caffeine, 1-beta-D-arabinofuranosylcytosine, and 5-fluorodeoxyuridine, on the expression of FRA3B.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1050285Documentos Relacionados
- Preferential integration of marker DNA into the chromosomal fragile site at 3p14: an approach to cloning fragile sites.
- A new folate sensitive fragile site at 1p21.3.
- Mapping of DNA markers close to the fragile site on the human X chromosome at Xq27.3.
- Sequence conservation at human and mouse orthologous common fragile regions, FRA3B/FHIT and Fra14A2/Fhit
- Two Breakpoint Clusters at Fragile Site FRA3B Form Phased Nucleosomes