The fission yeast genes pyp1+ and pyp2+ encode protein tyrosine phosphatases that negatively regulate mitosis.
AUTOR(ES)
Ottilie, S
RESUMO
We have used degenerate oligonucleotide probes based on sequences conserved among known protein tyrosine phosphatases (PTPases) to identify two Schizosaccharomyces pombe genes encoding PTPases. We previously described the cloning of pyp1+ (S. Ottilie, J. Chernoff, G. Hannig, C. S. Hoffman, and R. L. Erikson, Proc. Natl. Acad. Sci. USA 88:3455-3459, 1991), and here we describe a second gene, called pyp2+. The C terminus of each protein contains sequences conserved in the apparent catalytic domains of all known PTPases. Disruption of pyp2+ results in viable cells, as was the case for pyp1+, whereas disruption of pyp2+ and pyp1+ results in synthetic lethality. Overexpression of either pyp1+ or pyp2+ in wild-type strains leads to a delay in mitosis but is suppressed by a wee1-50 mutation at 35 degrees C or a cdc2-1w mutation. A pyp1 disruption suppresses the temperature-sensitive lethality of a cdc25-22 mutation. Our data suggest that pyp1+ and pyp2+ act as negative regulators of mitosis upstream of the wee1+/mik1+ pathway.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=360496Documentos Relacionados
- Negative regulation of mitosis in fission yeast by catalytically inactive pyp1 and pyp2 mutants.
- Isolation and characterization of the fission yeast protein phosphatase gene ppe1+ involved in cell shape control and mitosis.
- A ubiquitin-conjugating enzyme in fission yeast that is essential for the onset of anaphase in mitosis.
- Two fission yeast B-type cyclins, cig2 and Cdc13, have different functions in mitosis.
- A fission-yeast gene encoding a protein with features of protein-tyrosine-phosphatases.