The frameshift signal of HIV-1 involves a potential intramolecular triplex RNA structure
AUTOR(ES)
Dinman, Jonathan D.
FONTE
National Academy of Sciences
RESUMO
The cis-acting mRNA elements that promote programmed −1 ribosomal frameshifting present a natural target for the rational design of antiretroviral chemotherapies. It has been commonly accepted that the HIV-1 frameshifting signal is special, because its downstream enhancer element consists of a simple mRNA stem loop rather than a more complex secondary structure such as a pseudoknot. Here we present three lines of evidence, bioinformatic, structural, and genetic, showing that the biologically relevant HIV-1 frameshift signal contains a complex RNA structure that likely includes an extended RNA triple-helix region. We suggest that the potential intramolecular triplex structure is essential for viral propagation and viability, and that small molecules targeted to this RNA structure may possess antiretroviral activities.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=122769Documentos Relacionados
- Solution structure of the HIV-1 frameshift inducing stem–loop RNA
- The ability of the HIV-1 AAUAAA signal to bind polyadenylation factors is controlled by local RNA structure.
- Evolution of a disrupted TAR RNA hairpin structure in the HIV-1 virus.
- Dysregulation of signal transduction pathways as a potential mechanism of nervous system alterations in HIV-1 gp120 transgenic mice and humans with HIV-1 encephalitis.
- Accessibility of nuclear DNA to triplex-forming oligonucleotides: The integrated HIV-1 provirus as a target
