The G2 Checkpoint Is Maintained by Redundant Pathways
AUTOR(ES)
Passalaris, Tina M.
FONTE
American Society for Microbiology
RESUMO
While p53 activity is critical for a DNA damage-induced G1 checkpoint, its role in the G2 checkpoint has not been compelling because cells lacking p53 retain the ability to arrest in G2 following DNA damage. Comparison between normal human foreskin fibroblasts (HFFs) and HFFs in which p53 was eliminated by transduction with human papillomavirus type 16 E6 showed that treatment with adriamycin initiated arrest in G2 with active cyclin B/CDC2 kinase, regardless of p53 status. Both E6-transduced HFFs and control (LXSN)-transduced cells maintained a prolonged arrest in G2; however cells with functional p53 extinguished cyclin B-associated kinase activity. Down regulation was mediated by p53-dependent transcriptional repression of the CDC2 and cyclin B promoters. In contrast, cells lacking p53 showed a prolonged G2 arrest despite high levels of cyclin B/CDC2 kinase activity, at least some of which translocated into the nucleus. Furthermore, the G2 checkpoint became attenuated as p53-deficient cells aged in culture. Thus, at late passage, E6-transduced HFFs entered mitosis following DNA damage, whereas the age-matched parental HFFs sustained a G2 arrest. These results indicate that normal cells have p53-independent pathways to maintain DNA damage-induced G2 arrest, which may be augmented by p53-dependent functions, and that cells lacking p53 are at greater risk of losing the pathway that protects against aneuploidy.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=84436Documentos Relacionados
- DNA repair mutants defining G2 checkpoint pathways in Schizosaccharomyces pombe.
- p53 Regulation of G2 Checkpoint Is Retinoblastoma Protein Dependent
- A UV-responsive G2 checkpoint in rodent cells.
- PIAS-1 Is a Checkpoint Regulator Which Affects Exit from G1 and G2 by Sumoylation of p73
- p53 regulates a G2 checkpoint through cyclin B1