The Hha protein as a modulator of expression of virulence factors in Escherichia coli.
AUTOR(ES)
Mouriño, M
RESUMO
We constructed hha derivatives from both a clinical uropathogenic Escherichia coli isolate (strain FVL4) and a wild E. coli strain causing bovine diarrhea (strain CCB21) and analyzed the effect of the hha allele on the expression of the different virulence factors exhibited by these strains. Expression of hemolysin and of the Vir antigen was altered in hha mutants. Whereas production of hemolysin by strain FVL4 was repressed both at a low temperature and at high osmolarity, the hha allele accounted for a significant increase of hemolysin production under these conditions. Also, the low temperature-sensitive expression of the Vir adhesin was modified in hha mutants, which were able to express this adhesin at a low temperature. Expression of other virulence factors, such as cytotoxic necrotizing factor type 1 and 2 toxins, remained unmodified in hha derivatives of strains FVL4 and CCB21.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=174161Documentos Relacionados
- Expression of a proline-enriched protein in Escherichia coli.
- Alterations in Protein Expression Caused by the hha Mutation in Escherichia coli: Influence of Growth Medium Osmolarity
- Molecular epidemiology of adhesin and hemolysin virulence factors among uropathogenic Escherichia coli.
- Factors influencing inclusion body formation in the production of a fused protein in Escherichia coli.
- Cloning and expression of the Staphylococcus aureus protein A gene in Escherichia coli.
