The highly O-glycosylated glycoprotein gp2 of equine herpesvirus 1 is encoded by gene 71.

AUTOR(ES)

Wellington, J E

RESUMO

There have been conflicting reports regarding the gene assignment of the high-molecular-mass envelope glycoprotein gp2 (gp300) of equine herpesvirus 1. Here, we provide an unequivocal demonstration that gp2 is encoded by gene 71. gp2 that was purified with a defining monoclonal antibody was cleaved internally to yield a 42-kDa protein encoded by gene 71. Amino acid composition data and N-terminal sequence analysis of a tryptic peptide identified gp2 as the product of equine herpesvirus 1 gene 71 with the SWISS-PROT database. Analysis of gp2's monosaccharide composition and the 42-kDa subunit showed that the high level of O glycosylation occurs on the serine/threonine-rich region upstream of the cleavage site.

Documentos Relacionados

• The Truncated Form of Glycoprotein gp2 of Equine Herpesvirus 1 (EHV-1) Vaccine Strain KyA Is Not Functionally Equivalent to Full-Length gp2 Encoded by EHV-1 Wild-Type Strain RacL11
• The yeast cell fusion protein FUS1 is O-glycosylated and spans the plasma membrane.
• O-GLYCBASE version 2.0: a revised database of O-glycosylated proteins.
• O-GLYCBASE: a revised database of O-glycosylated proteins.
• Size Polymorphisms Due to Changes in the Number of O-Glycosylated Tandem Repeats in the Dictyostelium Discoideum Glycoprotein Psa