The HIV-1 Tat cellular coactivator Tat–SF1 is a general transcription elongation factor
AUTOR(ES)
Li, Xiao-Yong
FONTE
Cold Spring Harbor Laboratory Press
RESUMO
The human immunodeficiency virus type 1 (HIV-1) Tat protein strongly and specifically stimulates transcription elongation from the HIV-1 LTR and provides an important in vitro model system to study this process. Here we use protein-affinity chromatography to identify cellular factors involved in transcription elongation. A Tat-affinity column bound one transcription factor, Tat–SF1, efficiently and selectively. Tat–SF1 was identified originally as a Tat-specific coactivator, but we show it is a general transcription elongation factor. Our results also reveal the existence of an ATP-inactivatable general elongation factor (AIEF) required for Tat–SF1 activity and for which Tat can substitute functionally.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=317190Documentos Relacionados
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