The immediate-early mRNA that encodes the regulatory polypeptide Vmw 175 of herpes simplex virus type 1 is unspliced.

AUTOR(ES)

Rixon, F J

RESUMO

The structure and precise map location of the 4.1-kb herpes simplex virus type 1 (HSV-1) immediate-early mRNA (IE mRNA-3) that encodes a regulatory polypeptide, with an approximate mol. wt. of 175 000, has been determined. Nuclease S1 digestion procedures using labelled virus DNA probes have shown that IE mRNA-3 is unspliced and maps entirely within the TRs/IRs genome regions. DNA sequences at the 5' and 3' ends of IE mRNA-3 have been examined and regulatory signals involved in initiation of transcription and polyadenylation have been identified. No other mRNA is known to map within the region coding for IE mRNA-3.

Documentos Relacionados

• Separation and characterization of herpes simplex virus type 1 immediate-early mRNA's.
• Immediate-early mRNA-2 of herpes simplex viruses types 1 and 2 is unspliced: conserved sequences around the 5' and 3' termini correspond to transcription regulatory signals.
• Herpes simplex virus type 1 immediate-early protein Vmw110 reactivates latent herpes simplex virus type 2 in an in vitro latency system.
• Phenotype of a Herpes Simplex Virus Type 1 Mutant That Fails To Express Immediate-Early Regulatory Protein ICP0
• Orientation of herpes simplex virus type 1 immediate early mRNA's.