The influence of the purine 2-amino group on DNA conformation and stability. Synthesis and conformational analysis of d[T(2-aminoA)]3.

AUTOR(ES)

Gaffney, B L

RESUMO

A self-complementary hexanucleotide consisting of thymidine and 2-amino-deoxyadenosine, d(TA')3, has been synthesized by a solid phase phosphotriester method. Melting studies show that the additional hydrogen bond afforded by the 2-amino group substantially stabilizes the duplex. Moreover, conformational analysis using circular dichroism shows that a salt-induced conformational transition occurs, similar to the B leads to Z transition observed for d(CG)n oligonucleotides.

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