The isolation and characterization of bacteriophage T7 messenger RNA fragments containing an RNase III cleavage site.

AUTOR(ES)

Kramer, R A

RESUMO

We have isolated overlapping RNA fragments which contain the region surrounding the ribonuclease III cleavage site between bacteriophage T7 genes 0.3 and 0.7. Although all of these fragments contain the site of cleavage, only certain fragments are correctly recognized and cleaved by RNase III. Analysis of the cleavage products of the fragments indicates that the enzyme produces a single endonucleolytic break at this site in the T7 early RNA precursor molecule. In addition, the 3'-terminal adenylic acid residues observed previously on the in vivo T7 early RNA species were not found in these fragments and, therefore, must represent a post-transcriptional, post-processing modification of the RNA.

Documentos Relacionados

• Translation of T7 RNA in vitro without cleavage by RNase III.
• Effect of RNase III on the size of bacteriophage T7 lysozyme mRNA.
• Accurate in vitro cleavage by RNase III of phosphorothioate-substituted RNA processing signals in bacteriophage T7 early mRNA.
• Cleavage by RNase III Converts T3 and T7 Early Precursor RNA into Translatable Message
• Effect of RNase III on efficiency of translation of bacteriophage T7 lysozyme mRNA.