The lytE Gene of Bacillus subtilis 168 Encodes a Cell Wall Hydrolase
AUTOR(ES)
Margot, Philippe
FONTE
American Society for Microbiology
RESUMO
Bacillus subtilis cell wall-bound protein CWBP33 is encoded by lytE, a gene expressed during the exponential growth phase. Sequence analysis of LytE, a 33-kDa protein, reveals two domains. The N-terminal domain contains a threefold-repeated motif common to several peptidoglycan binding proteins, while the C-terminal domain, probably carrying the catalytic activity, has homology with certain exoproteins. Zymographs unambiguously reveal that the absence of CWBP33, due to inactivation of lytE, is accompanied by the loss of a lytic activity. In lytE mutants, the cell autolysis rate is significantly decreased, although autolysis of corresponding, purified cell walls does not seem to be affected.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=106949Documentos Relacionados
- The lytE Gene of Bacillus subtilis 168 Encodes a Cell Wall Hydrolase
- Localization of the Vegetative Cell Wall Hydrolases LytC, LytE, and LytF on the Bacillus subtilis Cell Surface and Stability of These Enzymes to Cell Wall-Bound or Extracellular Proteases
- Structural differentiation of the Bacillus subtilis 168 cell wall.
- Molecular cloning of a sporulation-specific cell wall hydrolase gene of Bacillus subtilis.
- Regulation of a New Cell Wall Hydrolase Gene, cwlF, Which Affects Cell Separation in Bacillus subtilis