The major RNA in prosomes of HeLa cells and duck erythroblasts is tRNA(Lys,3).
AUTOR(ES)
Nothwang, H G
RESUMO
Two-dimensional gel electrophoresis of HeLa cell prosomal RNAs, 3'-end labeled by RNA ligase, revealed one prominent spot. Determination of a partial sequence at the 3'-end indicated full homology to the 18 nucleotides at the 3'-end of tRNA(Lys,3) from rabbit, the bovine and the human species. An oligonucleotide complementary to the 3'-end of tRNA(Lys,3) hybridized on Northern blots with prosomal RNA from both HeLa cells and duck erythroblasts. In two-dimensional PAGE, the major pRNA of HeLa cells co-migrated with bovine tRNA(Lys,3). Reconstitution of the CCA 3'-end of RNA from both human and duck prosomes, by tRNA-nucleotidyl-transferase, confirmed the tRNA character of this type of RNA. Furthermore, it revealed at least one additional tRNA band about 85 nt long among the prosomal RNA from both species. Finally, confirming an original property of prosomal RNA, we show that in vitro synthesized tRNA(Lys,3) hybridizes stably to duck globin mRNA, and to poly(A)(+)- and poly(A)(-)-RNA from HeLa cells.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=312312Documentos Relacionados
- Synthesis and Processing of Nuclear Precursor-Messenger RNA in Avian Erythroblasts and HeLa Cells
- Stable tRNA precursors in HeLa cells.
- Interaction of HIV-1 reverse transcriptase with a synthetic form of its replication primer, tRNA(Lys,3).
- Active RNA polymerase I is fixed within the nucleus of HeLa cells.
- Internalization and recycling of CD4 transfected into HeLa and NIH3T3 cells.