The modulatory element TNNCT affects transcription of a Drosophila tRNA(Val)4 gene without affecting transcription complex stability.
AUTOR(ES)
Sajjadi, F G
RESUMO
We have previously identified a sequence of the general form TNNCT in the 5'-flanking region of a Drosophila melanogaster tRNA(Val)4 gene which positively modulates in vitro transcription. The pentadeoxynucleotide is also present in the 5'-flank of other Drosophila tRNA genes which direct transcription efficiently in vitro. We have examined transcription of a mutant of this gene further to understand the role of the TNNCT sequence. When template competition experiments were carried out, a template which contained mutations in TNNCT was a better competitor than was the wildtype template. The time-course of transcription and the effects of temperature and ionic strength on transcription indicated that mutating the TNNCT did not alter the efficiency of stable complex formation. It is proposed that the pentadeoxynucleotide TNNCT may influence the rate of transcription initiation by RNA polymerase III bound to transcription complexes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=331617Documentos Relacionados
- Transcription of a yeast telomere alleviates telomere position effect without affecting chromosome stability.
- The nucleotide sequence of the ferrochelatase and tRNA(val) gene region from Saccharomyces cerevisiae.
- Heterologous 5' flanking regions do not support in vitro template activity of a Drosophila melanogaster tRNA(Val3b) gene.
- The soybean chloroplast genome: nucleotide sequence of a region containing tRNA-Val (GAC) and 16S rRNA gene.
- Sequence of E.coli K12 valU operon which contains 3 genes for tRNA(Val1) and 1 gene for tRNA(Lys).
