The mur4 Mutant of Arabidopsis Is Partially Defective in the de Novo Synthesis of Uridine Diphospho l-Arabinose1
AUTOR(ES)
Burget, Emilie G.
FONTE
American Society of Plant Physiologists
RESUMO
To obtain information on the synthesis and function of arabinosylated glycans, the mur4 mutant of Arabidopsis was characterized. This mutation leads to a 50% reduction in the monosaccharide l-arabinose in most organs and affects arabinose-containing pectic cell wall polysaccharides and arabinogalactan proteins. Feeding l-arabinose to mur4 plants restores the cell wall composition to wild-type levels, suggesting a partial defect in the de novo synthesis of UDP-l-arabinose, the activated sugar used by arabinosyltransferases. The defect was traced to the conversion of UDP-d-xylose to UDP-l-arabinose in the microsome fraction of leaf material, indicating that mur4 plants are defective in a membrane-bound UDP-d-xylose 4-epimerase.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=59400Documentos Relacionados
- The Biosynthesis of l-Arabinose in Plants: Molecular Cloning and Characterization of a Golgi-Localized UDP-d-Xylose 4-Epimerase Encoded by the MUR4 Gene of Arabidopsis
- Production of Phenotypically Epimeraseless Yeast by l-Arabinose1
- l-Arabinose Negative Mutants of the l-Ribulokinase Structural Gene Affecting the Levels of l-Arabinose Isomerase in Escherichia Coli
- Positive Control of Enzyme Synthesis by Gene C in the l-Arabinose System
- Pentose metabolism in Mycobacterium smegmatis: comparison of L-arabinose isomerases induced by L-arabinose and D-galactose.