The occurrence of families of repetitive sequences in a library of cloned cDNA from human lymphocytes.
AUTOR(ES)
Crampton, J M
RESUMO
A library of cloned cDNAs representative of lymphocyte total poly(A)+ RNA was screened with total DNA probes at high clone density. 10% of the recombinants showed the presence of sequences which are repeated in the genome. Further analysis of six such isolated cDNA clones indicated that they contain different families of repetitive sequences with reiteration frequencies of between 150 and 45,000 copies per haploid genomes. Five of the six clones were found to contain single copy sequences as well as a repetitive sequence. cDNA clones containing repetitive sequences have been found to be derived from high, intermediate and low abundance classes of lymphocyte poly(A)+ RNA.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=327394Documentos Relacionados
- The construction of a recombinant cDNA library representative of the poly(A)+ mRNA population from normal human lymphocytes.
- The isolation of cloned cDNA sequences which are differentially expressed in human lymphocytes and fibroblasts.
- Nucleotide sequence of human cDNA coding for a lipoprotein lipase (LPL) cloned from placental cDNA library.
- Human M1 subunit of ribonucleotide reductase: cDNA sequence and expression in stimulated lymphocytes.
- Characterization of cloned cDNA sequences derived from Xenopus laevis poly A(+) oocyte RNA.