The origin of replication, oriC, and the dnaA protein are dispensable in stable DNA replication (sdrA) mutants of Escherichia coli K-12.

AUTOR(ES)

Kogoma, T

RESUMO

The sdrA224 mutants of Escherichia coli K-12, capable of continued DNA replication in the absence of protein synthesis (stable DNA replication), tolerate inactivation of the dnaA gene by insertion of transposon Tn10. Furthermore, oriC, the origin of E. coli chromosome replication, can be deleted from the chromosome of sdrA mutants without loss of viability. The results suggest the presence of a second, normally repressed, initiation system for chromosome replication alternative to the 'normal' dnaA+ oriC+-dependent initiation mechanism.

Documentos Relacionados

• The FIS protein binds and bends the origin of chromosomal DNA replication, oriC, of Escherichia coli.
• The FIS protein fails to block the binding of DnaA protein to oriC, the Escherichia coli chromosomal origin.
• Transcripts within the replication origin, oriC, of Escherichia coli.
• The DnaA box R4 in the minimal oriC is dispensable for initiation of Escherichia coli chromosome replication.
• Localized DNA melting and structural pertubations in the origin of replication, oriC, of Escherichia coli in vitro and in vivo.