The PRP31 gene encodes a novel protein required for pre-mRNA splicing in Saccharomyces cerevisiae.
AUTOR(ES)
Weidenhammer, E M
RESUMO
The pre-mRNA splicing factor Prp31p was identified in a screen of temperature-sensitive yeast strains for those exhibiting a splicing defect upon shift to the non- permissive temperature. The wild-type PRP31 gene was cloned and shown to be essential for cell viability. The PRP31 gene is predicted to encode a 60 kDa polypeptide. No similarities with other known splicing factors or motifs indicative of protein-protein or RNA-protein interaction domains are discernible in the predicted amino acid sequence. A PRP31 allele bearing a triple repeat of the hemagglutinin epitope has been generated. The tagged protein is functional in vivo and a single polypeptide species of the predicted size was detected by Western analysis with proteins from yeast cell extracts. Functional Prp31p is required for the processing of pre-mRNA species both in vivo and in vitro, indicating that the protein is directly involved in the splicing pathway.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=145753Documentos Relacionados
- Interactions of PRP2 protein with pre-mRNA splicing complexes in Saccharomyces cerevisiae.
- Six novel genes necessary for pre-mRNA splicing in Saccharomyces cerevisiae.
- PRP18, a protein required for the second reaction in pre-mRNA splicing.
- PRP38 encodes a yeast protein required for pre-mRNA splicing and maintenance of stable U6 small nuclear RNA levels.
- Identification of pre-mRNA polyadenylation sites in Saccharomyces cerevisiae.