The Reductive Inactivation of UDPgalactose-4-Epimerase from Yeast and E. coli*
AUTOR(ES)
Kalckar, Herman M.
RESUMO
The prosthetic groups of UDPgalactose-4-epimerase from yeast and E. coli can be reduced by incubation with D-galactose or L-arabinose provided that 5′ uridylic acid (5′UMP) is also present. This type of concerted reduction can also be performed using sodium borohydride. The latter type of reduction also depends on the addition of 5′UMP. Enzymatic analysis of the released prosthetic group by means of lactic dehydrogenase and pyruvate revealed that the pyridine nucleotide of the reduced epimerase generated in the concerted reaction is dehydrogenated in the 4-position of the pyridine ring.
