The Regulation of Protein Synthesis in Mammalian Cells VI. Soluble and Polyribosome Associated Components Controlling In Vitro Polypeptide Initiation in HeLa Cells*

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RESUMO

The in vitro initiation of polypeptides on endogenous polyribosomes has been studied in extracts from HeLa cells. Regulation of the rate of initiation of polypeptides can be examined. In these experiments an assay using [35S]fMet-tRNAfMet has been developed, and the system further characterized.

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