The Relation between Photophosphorylation and Delayed Light Emission in Chloroplasts 1

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RESUMO

One millisecond delayed light emission has been studied in isolated coupled lettuce (Lactuca sativa var. romaine) chloroplasts. Delayed light emission was increased upon addition of ferricyanide or 2,3,5,6-tetramethyl-p-phenylene diamine. In the presence of ferricyanide, the magnitude of the signal was increased by the addition of ADP (in the absence of orthophosphate), ATP, DI0-9, or phlorizin. The signal was also increased by the addition of NaCl and by the addition of NH4Cl in the presence of a high NaCl concentration. The signal of delayed light emission was decreased by the addition of gramicidin, valinomycin, and by the addition of NH4Cl in the presence of a low NaCl concentration.

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