The Riia Gene of Bacteriophage T4. II. Regulation of Its Messenger RNA Synthesis
AUTOR(ES)
Daegelen, P.
RESUMO
When the rII genes are first introduced into cells which had been previously infected by T4 phage deleted for these genes, the kinetics of synthesis of rIIA and rIIB RNA are rapid and identical. We show that this rapid synthesis depends on a functional motA gene for rIIB, but not for rIIA, RNA synthesis. By primer-extension mapping of T4 messenger RNA, we find three promoters close to the rIIA gene. One of them is an early promoter just before the rIIA.1 gene; it is used under all conditions tested. Another is in the coding portion of the rIIA.1 gene; it is weak, primarily because of a 19-bp spacing between the -10 and -35 elements, and its use is stimulated by T4 functions. The third is a motA-dependent (middle) promoter which has an unusual CCCGCTT box at -33. We present results which suggest that none of these promoters is likely to be the site at which the motB and motC gene products exercise their major influence on rIIA RNA synthesis.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1204015Documentos Relacionados
- Gene-specific mRNA. II. Regulation of mRNA synthesis in E. coli after infection with bacteriophage T4.
- Inhibition of Escherichia coli and bacteriophage lambda messenger RNA synthesis by T4.
- The Riia Gene of Bacteriophage T4. I. Its DNA Sequence and Discovery of a New Open Reading Frame between Genes 60 and Riia
- Properties of the nonlethal recombinational repair x and y mutants of bacteriophage T4. II. DNA synthesis.
- Enzymatic breakage and joining of deoxyribonucleic acid. II. The structural gene for polynucleotide ligase in bacteriophage T4.