The role of lactic acid in autocrine B-cell growth stimulation.
AUTOR(ES)
Pike, S E
RESUMO
Growth and survival of Epstein-Barr virus (EBV)-immortalized B lymphocytes cultured at low cell densities require autocrine soluble factors. In this study, we have purified a low molecular weight autocrine soluble factor that promotes growth of EBV-immortalized B cells in serum-free conditions and identified it as lactic acid (LA). Synthetic LA stimulated growth in EBV-immortalized B cells at 1-10 mM, a concentration of LA measured in the culture supernatant of EBV-immortalized cell lines. LA alone was found to account for greater than 70% of the autocrine growth factor activity in serum-free supernatants of EBV-immortalized B cells. Aminooxyacetate, a glutamate-oxaloacetate transaminase inhibitor, specifically inhibited B-cell growth induced by LA, suggesting that this process requires mitochondrial-cytosol transfers. Thus, LA is an autocrine stimulatory molecule that in serum-free conditions is essential for the continuous proliferation of EBV-immortalized B cells. This represents an unexpected function for LA.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=53077Documentos Relacionados
- Single cell studies on the role of B-cell stimulatory factor 1 in B-cell activation.
- B-cell growth factor: distinction from T-cell growth factor and B-cell maturation factor.
- T-cell-dependent B-cell stimulation is H-2 restricted and antigen dependent only at the resting B-cell level.
- Role of the Bp35 cell surface polypeptide in human B-cell activation.
- Role of ζPKC in B-cell signaling and function