The rpoN (sigma54) gene from Listeria monocytogenes is involved in resistance to mesentericin Y105, an antibacterial peptide from Leuconostoc mesenteroides.
AUTOR(ES)
Robichon, D
RESUMO
To gain insight into the mode of action of mesentericin Y105, a bacteriocin bactericidal agent against Listeria monocytogenes, we undertook to identify the listerial factors mediating this susceptibility by using a genetic approach. Transposon mutants resistant to the bacteriocin were obtained. One of them corresponded to a transposon insertion in a gene (rpoN) encoding a putative protein (447 amino acids) with strong homologies to alternative transcriptional sigma54 factors, including that of Bacillus subtilis (38% identity). Complementation experiments with the wild-type rpoN gene demonstrated that the insertion in rpoN was responsible for the resistance phenotype in L. monocytogenes. Moreover, expression of the L. monocytogenes rpoN gene in an rpoN mutant strain of B. subtilis promoted transcription of a sigma54-dependent operon in the presence of the associated regulator. These results demonstrate that the L. monocytogenes rpoN gene encodes a new sigma54 factor.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=179715Documentos Relacionados
- RpoN (sigma 54) is required for conversion of phenol to catechol in Acinetobacter calcoaceticus.
- Membrane permeabilization of Listeria monocytogenes and mitochondria by the bacteriocin mesentericin Y105.
- Bradyrhizobium japonicum has two differentially regulated, functional homologs of the sigma 54 gene (rpoN).
- The Requirement of RpoN (Sigma Factor ς54) in Denitrification by Pseudomonas stutzeri Is Indirect and Restricted to the Reduction of Nitrite and Nitric Oxide
- Mutational Analysis of Mesentericin Y105, an Anti-Listeria Bacteriocin, for Determination of Impact on Bactericidal Activity, In Vitro Secondary Structure, and Membrane Interaction