The spoIIIA locus is not a major determinant of prespore-specific gene expression during sporulation in Bacillus subtilis.

AUTOR(ES)

Illing, N

RESUMO

During sporulation in Bacillus subtilis, expression of several prespore-specific genes is strongly dependent on the spoIIIE and spoIIIG gene products. Previous reports have also indicated a requirement for the products of the spoIIIA locus. However, we have now systematically studied six different well-defined spoIIIA mutations and find that, relative to spoIIIE and spoIIIG mutations, they have only a minor effect on the expression of two different prespore-specific genes, spoVA and sspA. Moreover, we have shown that strain IS37, which has been used as a spoIIIA mutant in several previous studies, actually contains a lesion in the spo0A gene. We conclude that spoIIIA has a relatively minor or indirect role in the regulation of prespore-specific gene expression.

Documentos Relacionados

• Prespore-specific gene expression in Bacillus subtilis is driven by sequestration of SpoIIE phosphatase to the prespore side of the asymmetric septum
• Establishment of Prespore-Specific Gene Expression in Bacillus subtilis: Localization of SpoIIE Phosphatase and Initiation of Compartment-Specific Proteolysis
• Identification and Characterization of a New Prespore-Specific Regulatory Gene, rsfA, of Bacillus subtilis
• Analysis of the role of prespore gene expression in the compartmentalization of mother cell-specific gene expression during sporulation of Bacillus subtilis.
• Expression of a Bacillus megaterium sporulation-specific gene during sporulation of Bacillus subtilis.