The translational requirement for complete La Crosse virus mRNA synthesis is cell-type dependent.
AUTOR(ES)
Raju, R
RESUMO
The translational requirement to prevent premature termination during La Crosse virus S mRNA synthesis was found to be cell-type dependent. This requirement was present in the BHK, HEL, and Vero cell lines we examined, but not in C6/36 mosquito cells. The cell-dependent translational requirement could be reproduced in vitro by using either cell extracts or purified virions of BHK and C6/36 cells. In the BHK reactions, the polymerase terminated predominantly at nucleotide 175 in the absence of concurrent translation and required translation to read through this position. In the C6/36 reactions, however, the polymerase reads through nucleotide 175 efficiently independent of translation. Reconstitution studies suggested that the translational requirement was due to a factor(s) present in BHK, but not in C6/36, cells.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=251179Documentos Relacionados
- Translational requirement for La Crosse virus S-mRNA synthesis: a possible mechanism.
- Translational requirement of La Crosse virus S-mRNA synthesis: in vitro studies.
- Translational requirement of La Crosse virus S-mRNA synthesis: in vivo studies.
- La Crosse virus small genome mRNA is made in the cytoplasm.
- La Crosse virus infection of mammalian cells induces mRNA instability.