The wheat chloroplast gene for CF0 subunit I of ATP synthase contains a large intron
AUTOR(ES)
Bird, C. R.
RESUMO
The gene for CF0 subunit I of ATP synthase has been located in wheat chloroplast DNA, between the genes for CF0 subunit III and α subunit of CF1. Nucleotide sequencing and analysis of RNA-DNA hybrids indicated that the gene is interrupted by an 823-bp intron which has boundaries similar to those previously described for the introns in protein-coding chloroplast genes of Euglena gracilis. The deduced amino acid sequence of CF0 subunit I indicates a polypeptide of 183 amino acid residues. However, N-terminal amino acid sequencing of the mature spinach CF0 subunit I suggests that the protein is synthesised with a N-terminal extension of 17 amino acid residues and is processed to give a protein of mol. wt. 19 001 of 166 amino acids residues. The mature CF0 subunit I shows similarities in primary and predicted secondary structure to F0 subunit b of Escherichia coli ATP synthase. A major transcript of 3.3 kb containing sequences from the genes for CF0 subunit III, subunit I and CF1 subunit α has been observed by RNA-DNA hybridisation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=554355Documentos Relacionados
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