The yvaJ Gene of Bacillus subtilis Encodes a 3′-to-5′ Exoribonuclease and Is Not Essential in a Strain Lacking Polynucleotide Phosphorylase

AUTOR(ES)

Oussenko, Irina A.

FONTE

American Society for Microbiology

RESUMO

Studies of Bacillus subtilis RNases that are involved in mRNA degradation reveal a different pattern from that of Escherichia coli. A strain lacking polynucleotide phosphorylase, the major 3′-to-5′ exoribonuclease activity in cell extracts, is viable. Here, we show that the B. subtilis yvaJ gene encodes a second 3′-to-5′ exoribonuclease. A strain lacking both of these RNases grows slowly but is viable. The existence of another, as yet unknown, 3′-to-5′ exoribonuclease in B. subtilis is suggested.

Documentos Relacionados

• Properties of a Bacillus subtilis polynucleotide phosphorylase deletion strain.
• Protection against 3′-to-5′ RNA Decay in Bacillus subtilis
• tetA(L) Mutants of a Tetracycline-Sensitive Strain of Bacillus subtilis with the Polynucleotide Phosphorylase Gene Deleted
• Human Mitochondrial SUV3 and Polynucleotide Phosphorylase Form a 330-kDa Heteropentamer to Cooperatively Degrade Double-stranded RNA with a 3′-to-5′ Directionality*
• Bacillus subtilis YhaM, a Member of a New Family of 3′-to-5′ Exonucleases in Gram-Positive Bacteria