Thermal Inactivation of Staphylococcal Enterotoxins B and C1
AUTOR(ES)
Fung, Daniel Y. C.
RESUMO
Thermal inactivation profiles of staphylococcal enterotoxins B (SEB) and C (SEC) at 80, 100, and 121 C showed that SEC is more resistant than SEB to heat. After 24 h of incubation at 25 C, some reactivation (recovery of serological reactivity) occurred in toxins that had been inactivated by heat. If the toxin was stirred during heating, reactivation did not occur. An examination of the reactivation kinetics of heat-treated SEC showed that reactivation was temperature dependent. At 25 C, the incubation temperature of heat-treated crude SEC (80 C for 10 min), 100% reactivation occurred after 24 h, whereas at 4 C only slight reactivation was observed. We and others observed that heat-treated toxins initially lost more serological activity when heated at a low temperature (80 C) than at a higher temperature (100 C); in the present study we demonstrate that this is a reversible phenomenon.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=379937Documentos Relacionados
- Conservation of the biologically active portions of staphylococcal enterotoxins C1 and C2.
- Production of staphylococcal enterotoxins C1 and C2 and thermonuclease throughout the growth cycle.
- Residues 20, 22, and 26 determine the subtype specificities of staphylococcal enterotoxins C1 and C2.
- Scanning Density Gradient Isoelectric Focusing of Staphylococcus aureus Enterotoxins B and C1
- Production of Staphylococcal Enterotoxins A, B, and C in Various Media1
