Thermal Resistance of Pseudomonas fragi in Milk Containing Various Amounts of Fat1

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Similar populations of Pseudomonas fragi were grown at 25 C for 20 hr or at 7 C for 7 days in milk containing 0, 10, and 20% fat; they were then heated at 48, 50, and 52 C in milk containing 0, 10, and 20% fat. After inoculation, the heating medium contained 2.1 × 106 to 6.9 × 106 organisms per milliliter. The P. fragi cells grown in skim milk had greater thermal resistance (D52 = 3.0 to 3.1) than those grown in milk containing fat (D52 = 1.9 to 2.5). The organisms grown at 7 C for 7 days in milk containing 10% fat were more resistant (D52 = 3.0) than those grown in the same medium at 25 C for 20 hr (D52 = 2.0). The presence of 0 to 20% milk fat in the heating medium had no apparent effect on the thermal resistance.

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