Thermodynamic stability and drug-binding properties of oligodeoxyribonucleotide duplexes containing 3-deazaadenine:thymine base pairs.
AUTOR(ES)
Lever, C
RESUMO
We have used ultraviolet melting techniques to study the effect on stability of incorporating the nucleoside analogue 2'-deoxy-3-deazaadenosine (d3cA) into the duplex 5'-d(CGCAATCG)-3'-d(GCGTTAGC). Our results demonstrate that the successive replacement of dA by d3CA increasingly destabilises the duplex. The destabilising effect of this analogue is considerably enhanced as the pH is lowered and the results are consistent with protonation of 3-deazaadenine (probably at N-1) contributing to duplex destablisation. Surprisingly, the incorporation of d3CA does not significantly affect the binding of distamycin-A.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=309409Documentos Relacionados
- Characterization of imperfect DNA duplexes containing unpaired bases and non-Watson-Crick base pairs.
- Dissociation kinetics of 19 base paired oligonucleotide-DNA duplexes containing different single mismatched base pairs.
- Pharmaceutical-grade albumin: impaired drug-binding capacity in vitro
- Vacuum UV CD spectra of homopolymer duplexes and triplexes containing A.T or A.U base pairs.
- CD of homopolymer DNA-RNA hybrid duplexes and triplexes containing A-T or A-U base pairs.