Three myosin V structures delineate essential features of chemo-mechanical transduction
AUTOR(ES)
Coureux, Pierre-Damien
FONTE
Nature Publishing Group
RESUMO
The molecular motor, myosin, undergoes conformational changes in order to convert chemical energy into force production. Based on kinetic and structural considerations, we assert that three crystal forms of the myosin V motor delineate the conformational changes that myosin motors undergo upon detachment from actin. First, a motor domain structure demonstrates that nucleotide-free myosin V adopts a specific state (rigor-like) that is not influenced by crystal packing. A second structure reveals an actomyosin state that favors rapid release of ADP, and differs from the rigor-like state by a P-loop rearrangement. Comparison of these structures with a third structure, a 2.0 Å resolution structure of the motor bound to an ATP analog, illuminates the structural features that provide communication between the actin interface and nucleotide-binding site. Paramount among these is a region we name the transducer, which is composed of the seven-stranded β-sheet and associated loops and linkers. Reminiscent of the β-sheet distortion of the F1-ATPase, sequential distortion of this transducer region likely controls sequential release of products from the nucleotide pocket during force generation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=533045Documentos Relacionados
- Chemo-mechanical energy transduction in relation to myosin isoform composition in skeletal muscle fibres of the rat.
- Randomized controlled clinical trial of long-term chemo-mechanical caries removal using PapacarieTM gel
- Avaliação clínica de dois métodos minimamente invasivos: químico-mecânico e mecânico (ART)
- Radiographic and microbiological analysis of root canals of dogs teeth with periapical lesions before and after chemo-mechanical preparation
- Avaliação de alterações comportamentais e fisiológicas durante a remoção de tecido cariado através dos métodos mecânico e químico-mecânico (CARISOLV TM ) em crianças com Síndrome de Down.