Tn4563 transposition in Streptomyces coelicolor and its application to isolation of new morphological mutants.
AUTOR(ES)
Schauer, A T
RESUMO
The Tn3-like transposon Tn4556 (and its derivatives Tn4560 and Tn4563) has been used for insertion mapping of genetic loci cloned on plasmids, but it has been difficult to obtain chromosomal insertions, largely because of the lack of a strong selection against transposon donor molecules. In this communication, we report two efficient selection techniques for transposition and their use in the isolation of chromosomal insertion mutations. A number of independent Streptomyces coelicolor morphological mutants (bld and whi) were obtained. Two of the bld mutations were mapped to locations on the chromosome by SCP1-mediated conjugation; at least one mutation, bld-5m1, appears to define a novel locus involved in control of S. coelicolor morphogenesis and antibiotic production.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=208196Documentos Relacionados
- Transposon Tn554: complete nucleotide sequence and isolation of transposition-defective and antibiotic-sensitive mutants.
- Changes in patterns of ADP-ribosylated proteins during differentiation of Streptomyces coelicolor A3(2) and its development mutants.
- New loci required for Streptomyces coelicolor morphological and physiological differentiation.
- Isolation of tetracenomycin C-nonproducing Streptomyces glaucescens mutants.
- Isolation of Bacteroides fragilis Mutants with In Vivo Growth Defects by Using Tn4400′, a Modified Tn4400 Transposition System, and a New Screening Method