Tn5-mediated transposition of plasmid DNA after transduction to Myxococcus xanthus.
AUTOR(ES)
Downard, J S
RESUMO
After coliphage P1-mediated transfer of Tn5-containing plasmid DNA from Escherichia coli to Myxococcus xanthus, transductants were identified which contained plasmid sequences integrated at many sites on the bacterial chromosome. The unaltered plasmid DNA sequences in these transductants were apparently flanked by intact Tn5 or IS50 sequences. These results suggest that Tn5-mediated transposition has occurred and provide a method for integrating plasmid DNA into the M. xanthus chromosome without the requirement for homologous recombination.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=211541Documentos Relacionados
- Defects in fruiting body development caused by Tn5 lac insertions in Myxococcus xanthus.
- Host-dependent transposon Tn5-mediated streptomycin resistance.
- Tactic behavior of Myxococcus xanthus.
- Construction of Tn5 lac, a transposon that fuses lacZ expression to exogenous promoters, and its introduction into Myxococcus xanthus.
- Extracellular fibrils and contact-mediated cell interactions in Myxococcus xanthus.
