TnpA product encoded by the transposable element En-1 of Zea mays is a DNA binding protein.

AUTOR(ES)

Gierl, A

RESUMO

TnpA protein, the function encoded by the most abundant transcript of En-1 was expressed in Escherichia coli. DNA binding experiments with partially purified tnpA protein revealed that it binds to the subterminal repetitive region of En-1. TnpA protein recognizes a 12-bp-long sequence motif which is reiterated several times at the termini of En-1. Binding is reduced if the cytosine residues of CG dinucleotides and CNG trinucleotides within the motif are methylated. These data suggest a model in which the product of tnpA serves as a regulator of element activity.

Documentos Relacionados

• Inducible DNA Demethylation Mediated by the Maize Suppressor-mutator Transposon-Encoded TnpA Protein
• Excision of the En/Spm transposable element of Zea mays requires two element-encoded proteins.
• En/Spm encoded tnpA protein requires a specific target sequence for suppression.
• The defective En-I102 element encodes a product reducing the mutability of the En/Spm transposable element system of Zea mays
• Genetic and molecular analysis of the Enhancer (En) transposable element system of Zea mays