Transcription and replication initiate at separate sites on the vesicular stomatitis virus genome
AUTOR(ES)
Whelan, Sean P. J.
FONTE
The National Academy of Sciences
RESUMO
The RNA-dependent RNA polymerase of the nonsegmented negative-strand RNA viruses carries out two distinct RNA synthetic processes: transcription of monocistronic, capped, and polyadenylated subgenomic messenger RNAs, and replication by means of the synthesis of a full-length positive-sense copy of the genome. The template for both processes is the negative-sense genomic RNA tightly encapsidated by the viral nucleocapsid protein. By applying UV transcriptional mapping to engineered variants of vesicular stomatitis virus, we discovered that, in infected cells, transcription and replication are controlled by initiation at different positions on the viral genome.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=123114Documentos Relacionados
- Overlapping Signals for Transcription and Replication at the 3′ Terminus of the Vesicular Stomatitis Virus Genome
- RNA Synthesis of Vesicular Stomatitis Virus V. INTERACTIONS BETWEEN TRANSCRIPTION AND REPLICATION
- Two RNA polymerase complexes from vesicular stomatitis virus-infected cells that carry out transcription and replication of genome RNA
- Two separate domains within vesicular stomatitis virus phosphoprotein support transcription when added in trans.
- Phosphorylation sites on phosphoprotein NS of vesicular stomatitis virus.
