Transcription factor MBF-I interacts with metal regulatory elements of higher eucaryotic metallothionein genes.
AUTOR(ES)
Imbert, J
RESUMO
Metallothionein (MT) gene promoters in higher eucaryotes contain multiple metal regulatory elements (MREs) that are responsible for the metal induction of MT gene transcription. We identified and purified to near homogeneity a 74-kilodalton mouse nuclear protein that specifically binds to certain MRE sequences. This protein, MBF-I, was purified employing as an affinity reagent a trout MRE that is shown to be functional in mouse cells but which lacks the G+C-rich and SP1-like sequences found in many mammalian MT gene promoters. Using point-mutated MREs, we showed that there is a strong correlation between DNA binding in vitro and MT gene regulation in vivo, suggesting a direct role of MBF-I in MT gene transcription. We also showed that MBF-I can induce MT gene transcription in vitro in a mouse extract and that this stimulation requires zinc.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=363696Documentos Relacionados
- A nuclear factor binds to the metal regulatory elements of the mouse gene encoding metallothionein-I.
- Metal-specific posttranscriptional control of human metallothionein genes.
- A zinc-responsive factor interacts with a metal-regulated enhancer element (MRE) of the mouse metallothionein-I gene.
- The C-terminal domain of transcription factor IIIA interacts differently with different 5S RNA genes.
- Interaction of the CCAAT displacement protein with shared regulatory elements required for transcription of paired histone genes.