Transcription of Bacteriophage PM2 Involves Phage-Encoded Regulators of Heterologous Origin
AUTOR(ES)
Männistö, Riina H.
FONTE
American Society for Microbiology
RESUMO
Bacteriophage PM2 is the only described member of the Corticoviridae family. It is an icosahedral dsDNA virus with a membrane residing underneath the protein coat. PM2 infects some gram-negative Pseudoalteromonas spp. In the present study, we mapped the viral promoters and showed that the PM2 genome consists of three operons. Four new virus genes were assigned based on their function in transcription. Proteins P15 and P16 are shown to repress early transcription, and proteins P13 and P14 are shown to activate late transcription events. The early regulatory region, containing genes for proteins P15 and P16, as well as the newly identified early promoter region in PM2, has significant sequence similarity with the Pseudoalteromonas pAS28 plasmid. P14, the transcription activator for the structural genes, has a zinc finger motif homologous to archaeal and eukaryotic TFIIS-type regulatory factors.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=155381Documentos Relacionados
- Origin of Phospholipid in Bacteriophage PM2
- Molecular Characterization of a Phage-Encoded Resistance System in Lactococcus lactis
- A satellite phage-encoded antirepressor induces repressor aggregation and cholera toxin gene transfer
- The In Vitro Interaction of Streptococcus pyogenes with Human Pharyngeal Cells Induces a Phage-Encoded Extracellular DNase
- Properties and Characterization of the Host Bacterium of Bacteriophage PM2