Transcription of the human corticotropin-releasing hormone gene in NPLC cells is correlated with Z-DNA formation.
AUTOR(ES)
Wölfl, S
RESUMO
The intron of the corticotropin-releasing hormone (corticoliberin; CRH) gene contains a sequence of over 100 bp of alternating purine/pyrimidine residues. We have used binding of a Z-DNA-specific antibody in metabolically active, permeabilized nuclei to study the formation of Z-DNA in this sequence at various levels of transcription. In the NPLC human primary liver carcinoma cell line, activation of cAMP-dependent pathways increased the level of transcription, while adding glucocorticoids inhibited transcription of the CRH gene. These cells respond in a manner similar to hypothalamic cells. Z-DNA formation in this sequence was detected at the basal level of transcription, as well as after stimulation with forskolin. Inhibition of transcription by dexamethasone abolished Z-DNA formation. Z-DNA formation in the WC gene (c-myc) was affected differently in the same experiment. Thus, changes in Z-DNA formation in the CRH gene are gene specific and are linked to the transcription of the gene.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=39668Documentos Relacionados
- Glucocorticoid stimulates expression of corticotropin-releasing hormone gene in human placenta.
- Impaired diurnal adrenal rhythmicity restored by constant infusion of corticotropin-releasing hormone in corticotropin-releasing hormone-deficient mice.
- Z-DNA formation in the rat growth hormone gene promoter region.
- Corticotropin-releasing hormone: An autocrine hormone that promotes lipogenesis in human sebocytes
- Immunoreactive corticotropin-releasing factor in human plasma.