Transcription of the nonrepeated fraction of "accessible" DNA in rat liver chromatin.
AUTOR(ES)
Arnold, E A
RESUMO
The relationship between structure and function in eukaryotic chromatin has been studied in rat liver cells. To elucidate the functional significance of "accessible" DNA, the transcription of this DNA (prepared by titration of liver chromatin with poly-D-lysine) has been examined by RNA-DNA hybridization. The maximum extent to which nuclear RNA will hybridize to the nonrepeated fraction of "accessible" DNA has been measured and compared to the extent that whole chromatin DNA will hybridize. The results show that "accessible" DNA has the same number of sequences complementary to nuclear RNA as does total DNA. In addition DNA-DNA reassociation experiments indicate that there is only a small difference between the total unique sequence populations of "accessible" and total DNA. These results indicate that nonrepeated "accessible" DNA is not preferentially transcribed in the cell as is predicted by some models of chromatin structure.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=342902Documentos Relacionados
- Organization of internucleosomal DNA in rat liver chromatin.
- Physical properties of chemically acetylated rat liver chromatin.
- In vitro transcription of vitellogenin sequences on chick liver chromatin.
- Isolation and characterization of the DNA fraction of rat liver chromatin which binds polylysine.
- Template activities in normal, regenerating, and developing rat liver chromatin.
