Transcription of the Polyoma Virus Genome: Synthesis and Cleavage of Giant Late Polyoma-Specific RNA
AUTOR(ES)
Acheson, Nicholas H.
RESUMO
The size of virus-specific RNA synthesized in cultured mouse kidney cells infected with polyoma virus was estimated by electrophoresis and sedimentation analysis of RNA extracts from whole cells. Newly synthesized “late” polyoma-specific RNA appears as “giant” molecules of heterogeneous size, up to several times larger than a strand of polyoma DNA (1.5 × 106 daltons). Treatment with dimethylsulfoxide or urea showed that the large size of these molecules is not due to aggregation. Giant polyoma-specific RNA is strikingly similar in size distribution to “nuclear messenger-like” RNA (“heterogeneous nuclear” RNA) of the host cell. Subsequent to its synthesis, some of the giant polyoma-specific RNA appears to be cleaved to at least three smaller species.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=389391Documentos Relacionados
- Isolation and characterization of poly(A)-containing intranuclear polyoma-specific "giant" RNA'S.
- Studies of polyoma virus DNA: cleavage map of the polyoma virus genome.
- Polyoma virus-specific RNA synthesis in an inducible line of polyoma virus-transformed rat cells.
- Mapping of Early and Late Transcripts Encoded by the Autographa californica Nuclear Polyhedrosis Virus Genome: Is Viral RNA Spliced?
- Transcription of the JC virus archetype late genome: importance of the kappa B and the 23-base-pair motifs in late promoter activity in glial cells.
