Transcription termination in the archaebacterium Sulfolobus: signal structures and linkage to transcription initiation.

AUTOR(ES)

Reiter, W D

RESUMO

The precise map positions were determined for the 3'-termini of five transcripts of the Sulfolobus virus-like particle SSV1. In all cases analyzed, these 3'-termini mapped immediately downstream of a sequence TTTTTYT which was part of a pyrimidine-rich region of 16-19 nucleotides length. No correlation was evident between the position of the 3'-termini and possible secondary structures within the RNA. In two cases, the 3'-termini of SSV1 transcripts mapped in the immediate vicinity of transcriptional initiation sites suggesting that transcription termination can be linked to the re-initiation of RNA synthesis.

Documentos Relacionados

• In vitro transcription of two rRNA genes of the archaebacterium Sulfolobus sp. B12 indicates a factor requirement for specific initiation.
• Mutation of a termination codon affects src initiation.
• Analysis of transcription in the archaebacterium Sulfolobus indicates that archaebacterial promoters are homologous to eukaryotic pol II promoters.
• A plasmid in the archaebacterium Sulfolobus acidocaldarius
• Three replication origins in Sulfolobus species: Synchronous initiation of chromosome replication and asynchronous termination