Transduction and transformation of plasmid DNA in Streptomyces fradiae strains that express different levels of restriction.

AUTOR(ES)

Matsushima, P

RESUMO

We constructed nonrestricting strains of Streptomyces fradiae blocked in different steps in tylosin biosynthesis. Plasmid transformation frequencies were 10(3)- to 10(4)-fold higher and bacteriophage plating efficiencies were 10(4)- to 10(8)-fold higher in the nonrestricting strains than in the restricting strains. The efficiencies of transduction of plasmid pRHB101 in S. fradiae strains varied by over 1,000-fold, depending on growth conditions, and optimum transduction frequencies were obtained when cells were grown to mid-exponential phase at 39 degrees C. Under these conditions, restricting and nonrestricting strains were transduced at frequencies that differed by only two- to fivefold.

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