Transfection of human lymphoblastoid cells with herpes simplex viral DNA.
AUTOR(ES)
Miller, G
RESUMO
The "calcium/dimethyl sulfoxide shock" method of transfection was adapted for use in human lymphoid cell cultures. One microgram of herpes simplex virus type 1 DNA regularly initiated virus replication in four lymphoblastoid cell lines. Per 10(5) cells exposed to 1 microgram of DNA, 0.5--5 cells formed an infectious center. The minimal infective dose of DNA was approximately 500 ng.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=383102Documentos Relacionados
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